ABSTRACT
Objective To observe the effect of different hemodialysis methods on calcium and phosphorus metabolism in uremic patients .Methods 130 patients with uremia who underwent hemodialysis were divided into maintenance hemodialysis group ( HD group ) and maintenance hemodialysis filtration group ( HDF group ) according to different dialysis methods ,65 cases in each group .The HD group was treated with maintenance hemodialysis .The HDF group received high -throughput polysulfone membrane dialyzer ,the two groups received dialysis for 6 months. The Ca2+,serum phosphorus (P3+),parathyroid hormone (iPTH),1,25 dihydroxyvitamin D[1,25(OH)2D] were measured before and after treatment in both two groups .Results The total effective rate was 76.92％ in the HD group and 84.62％in the HDF group.There was no statistically significant difference between the two groups (χ2 =1.25,P=0.535).After treatment,Ca2+in the two groups was statistically significantly increased compared with before treatment(t=4.841,P=0.00;t=8.600,P=0.00),and Ca2+in the HDF group was higher than that in the HD group (t=4.410,P=0.00).After treatment,P3+in the two groups was significantly decreased compared with before treatment(t=14.580,P=0.00;t=19.260,P=0.00),and P3+in the HDF group was lower than that in the HD group(t=6.500,P=0.00).After treatment,iPTH in the two groups was significantly decreased compared with before treatment(t=58.800,P=0.00;t=65.730,P=0.00),and iPTH in the HDF group was significantly lower than that in the HD group (t=8.380,P=0.00).After treatment,the 1,25(OH)2D levels in the two groups were significantly higher than those before treatment (t=18.970,P=0.00;t=21.650,P=0.00),and 1,25(OH)2D level in HDF group was significantly higher than HD group (t=3.250,P=0.001).Conclusion Compared with maintenance hemodialysis , maintenance hemodialysis filtration has more positive effect on calcium and phosphorus metabolism in uremic patients ,and it has significant effect on lowering blood phosphorus and increasing serum calcium level,so it is more suitable for clinical use .
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method to determine total bromine in urine.</p><p><b>METHOD</b>Diluted urine samples were directly introduced into ICP-MS then quantized by standard curve.</p><p><b>RESULT</b>Total bromine in urine was linear within 1.0~50 mg/L with r > 0.999, When spiked at a concentration of 0.020 mg/L, 0.050 mg/L, 0.150 mg/L, the recovery was 95%~98%, intra-assay precision was 1.4% 3.2%, inter-assay precision was 3.4% to 5.0%. Urine could store in -20 °C refrigerator 3 months without any bromine loss.</p><p><b>CONCLUSION</b>Using ICP-MS to determine the urinary total bromine, the method is fast, accurate, wide linear range of features, could meet with the requirement of Part 5 of occupational health standards guide: Method determination of chemical substances in biological materials (GBZ/T 210.5-2008), a strong competitive advantage in a wide range of survey, suitable for promotion.</p>